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Care and Use information

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Evervoir96ä Standard and UV Sensitive versions

This is a recirculating low volume reservoir for use with various automated liquid handlers and manual laboratory use. Use this reservoir to maintain continuous levels from a 500 mL resource vessel for extended assay operations.A 96 channel lid is also included to minimize evaporation and to allow for tip touching

Automated Use:Define the reservoir along with it’s lid using the respective software utilities for your Liquid handler.To do this refer to the following dimensions. *Tip: the well access locations , Length and width dimensions are the same as a standard 96 well microplate.

Dimensions:

Minivoir96 Height: ……….. 19 mm

Max trough Depth:……….. 16.75mm (24.75mm lid on)

Lid on Height:……………... 26mm

Lid well Diameter: ………... 6.5mm

Max Tip-Touch Depth: …… 9.5mm (64-100% of total height)

X wide: ……………………... 127.6mm

Y narrow: ………………….. 85.8mm

A1 to -X edge: …………… 14.25mm (96well flat locations)

A1 to -Y edge: ……………. 11.32mm

Specifications:

Refill rate, Adjustable..…… 1000uL to 10mL / Minute

Reservoir Level, Mode 1:… 15ml

Reservoir Level, Mode 2:… 130ml

Material: reservoir…………. Polypropylene

Material: Tubing……………. TygonÔ

Material: Bottle..…………… Polyethylene

Peristaltic Pump…………… CSA, CE, UL 12vdc with 115VAC supply

Installation:For the best operational performance, the reservoir base must remain as flat and level as possible.The fill tubing ID is always to be the smaller , thus the drain tubing is always to be the Larger ID.The small bore tubing must be coaxed onto the barb. Patience please. The bottle openings are sized for the tubing. The > symbol is for the larger tubing. Minimize the tubing length to reduce the dead volume.
Mode 1: Fill tubing is connected to the upper connection. 15 mL Level
Mode 2: Fill tubing is connected to the bottom connection. 130 mL Level

Operation:Fill the bottle with reagent. Insert the tubing and route to avoid sharp bends. Be certain that the fill tubing is the smaller tube and that the direction of rotation is appropriate (clockwise is typical but dependant on the tube routing). Plug the power Cable into the back of the C/L pump ( green LED should be on). Depress and hold the MAX button on the controller to prime the reservoir. Once the surface has been wetted then install the EvaprCapÔ . It is safe to proceed when the reservoir has enough initial capacity for you assay.

Clean-up

Chemical Sterilization: Use of most commonly available disinfectants can be used. Avoid prolonged exposure to the more aggressive disinfectants to avoid any surface cracking.

Autoclaving: Rinse clean in DI water prior to periods of autoclaving.

Do not autoclave polypropylene LID

Remove port fittings before procedure
121° C / 15 psi. Cycle length of 15 minutes initial duration.

The cycle period varies according to the type of chemical / material exposure.

Proper periods can only be determined with experience.

If you encounter any difficulty with this device or require custom modifications feel free to

Contact: JeffCahlik@ACME-Automation or US 423 452 0893.

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