Contact

             click to return to Turbulator2 product sheet

Automated Use:Define the TurbulatorÔ using the respective software utilities for your Liquid handler.To do this refer to the following dimensions. *Tip: the well access locations , Length and width dimensions are the same as a standard 96 well microplate.

Operation:The device is to be connected to a large diaphragm vacuum pump only.Recommended pump Model Gast DOA-P104-AA. Other large diaphragm pumps can be used.The function of the pump is to create a vacuum pulse. Actual pump capacity or ultimate vacuum pressure is not the goal. The vacuum pulse reduces the pressure at the Turbulator diaphragm and draws a small volume of liquid under the reservoir base through the orifices. When the vacuum pulse subsides the pressure is allowed to equalize. The diaphragm and return packing, force the aspirated sample up through the orifices again. This recursive process creates a stirring action that is sufficient to maintain suspension of most solids in solution. By adjusting a bleeder valve at the pump the influence on the Turbulator diaphragm can be controlled. DO NOT block the open port on the Turbulator, It will force it to aspirate a large volume and once released it will launch 96 little geysers into your face. A slight tap on the port opening can (prime) initiate mixing on some very viscous fluids.
Dimensions:

Operation:For the best operational performance, the reservoir base must remain as flat and level as possible. DO not fill above 130ml or the lid installation will spill some of the contents. Once well depth and position have been properly determined you can supply liquids. It is best to agitate the liquids before pouring to break up the solids and resuspend them. Fill the reservoir area. Immediately turn on the pump. The surface should appear turbulent and mixing will begin. A small volume may be unavailable to reservoir to provide mixing. If your vacuum bleeder is fully closed and you believe the diaphragm is not primed very briefly tap closed the open port on the Turbulator. This should momentarily induce a turbulent event. If mixing is not vigorous enough it may be required that a thinner membrane be used. Contact ACME-Automation.com

Diaphragm Replacement: It may become required that you replace the diaphrgam for cleaning. The technique is to place a new diaphragm on the back of a moist reservoir first. Then apply even downward force with the base for the entire period you are screwing down the the base . Early units may be marked with a index scribe to orient the base with the reservoir. Tighten the screws until they have bottomed out, release pressure. Test operation using DI water to check for leaks. Your unit is now ready for use.

1. When you are finished with a run either replenish and keep the vacuum on or empty the reservoir.

Once the reservoir has been emptied, rinse repeatedly with DI water and sterilize see below.

The units should not be disassembled without a spare diaphragm .Contact Jeff Cahlik at ACME-Automation 423-452-0893. Replacement diaphragms are available in various thicknesses as this is a consumable item.

2. Chemical Sterilization: Use of most commonly available disinfectants can be used. Avoid prolonged exposure to the more aggressive disinfectants to avoid any surface cracking.
3. Autoclaving: Can only be done to units which have been disassembled, Rinse clean in DI water prior to periods of autoclaving. Do not autoclave LID
   121° C / 15 psi. Cycle length of 10 minutes minimum duration. The cycle period varies according to the type of chemical / material exposure. Proper periods can only be determined with experience.